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Effects of Dictamnus dasycarpus Turcz., root bark on ICAM-1 expression and chemokine productions in vivo and vitro study

Authors Han, Hye-Yeon; Ryu, Mi Heon; Lee, Guemsan; Cheon, Won-Ju; Lee, Chu; An, Won-Gun; Kim, Hyungwoo; Cho, Su-In

Journal JOURNAL OF ETHNOPHARMACOLOGY , JAN 15 2015,:159

DOI 10.1016/j.jep.2014.11.020 PubMed 25449455

Abstract Ethnopharmacological relevance: The root bark of Dictamnus dasycarpus Turcz., family Rutaceae is a well known anti-inflammatory agent for skin diseases such as eczema, pruritus and urticaria in Eastern countries.Materials and methods: We investigated the effects of methanol extract of Dictamnus dasycarpus root bark (MEDD) on Intercellular Adhesion Molecule-1 (ICAM-1) expression, epidermal hyperplasia and immune cell infiltration in 1-fluoro-2,4-dinitrofluorobenzene (DNFB)-induced contact dermatitis (CD) mice. We also investigated its effects on the expression of ICAM-1, binding capacity to THP-1 cells, cytokine and chemokine production, and phosphorylation of NF-kappa B in human keratinocytes (HaCaT cells).Results: Topical application of MEDD effectively inhibited ICAM-1 expression and epidermal hyperplasia in inflamed tissues. MEDD treatment also inhibited immune cell infiltration induced by DNFB. In addition, treatment with MEDD reduced surface expression and total amount of ICAM-1 in HaCaT cells and effectively lowered the capacity to bind to THP-1 cells. MEDD also lowered the levels of IL-6, IL-8, monokine induced by gamma interferon (MIG), monocyte chemotactic protein-1 (MCP-1) and regulated on activation, normal T cell expressed and secreted (RANTES). Finally, MEDD treatment prevented activation of the NF-kappa B pathway induced by TNF-alpha in HaCaT cells.Conclusions: These data indicate that root bark of Dictamnus dasycarpus has the potential for treatment of inflammatory skin diseases as a complementary or alternative medicine to corticosteroids. In addition, they suggest that the anti-inflammatory effects of Dictamnus dasycarpus on CD are involved in the regulation of ICAM-1 expression and cytokine and chemokine secretion through down-regulation of the NF-kappa B signaling pathway in keratinocytes. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

Species covered 白鲜 Dictamnus dasycarpus