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Anti-hepatitis B virus activity of swertisin isolated from Iris tectorum Maxim

Authors Xu, Hong-Yan; Ren, Ji-Hua; Su, Yu; Ren, Fang; Zhou, Yu-Jiao; Jiang, Hui; Cheng, Sheng-Tao; Zhang, Chuan-Rui; Chen, Juan

Journal JOURNAL OF ETHNOPHARMACOLOGY , JUL 15 2020,:257

DOI 10.1016/j.jep.2020.112787 PubMed 32224198

Abstract Ethnopharmacological relevance: Iris tectorum Maxim (I. tectorum, Yuan Wei in Chinese) is a common and traditional Chinese medicinal herb that be used to treat liver-related diseases. However, the anti-HBV activity of I. tectorum and its isolates has not been systemically studied.Aim of the study: To screen the active part of I. tectorum and systemically evaluate their anti-HBV activity.Materials and methods: In this study, a series of compounds from I. tectorum were evaluated for their ability to inhibit HBV replication. Swertisin showed a significant inhibitory function on HBV replication. Then, the suppression effect of different concentrations of swertisin in HBsAg, HBeAg and HBV DNA level in HepG2.2.15 cells and HBV-infected HepG2-NTCP cells were comprehensive evaluated, respectively. Moreover, the anti-HBV effects of swertisin were confirmed in HBV transgenic mice model.Results: Among these compounds, swertisin strongly inhibited the HBsAg, HBeAg and HBV DNA level in a dose-dependent manner in HepG2.2.15 cells and HBV-infected HepG2-NTCP cells. Furthermore, swertisin showed a significant inhibition role on HBV replication in HBV transgenic mice model, the inhibition effect of which was enhanced when combined with ETV.Conclusions: We have identified that swertisin can inhibit HBeAg and HBsAg production, as well as HBV DNA in vitro and in vivo. This study show that we may found a novel compound isolated from traditional Chinese medicines with potent anti-HBV function.

Species covered 鸢尾 Iris tectorum