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Comparative HPLC analysis of phenolic compounds in the roots of Acanthopanax trifoliatus and Acanthopanax senticosus

Authors Sithisarn, P.; Jarikasem, S.; Muensaen, S.

Journal XXIX INTERNATIONAL HORTICULTURAL CONGRESS ON HORTICULTURE:SUSTAINING LIVES,LIVELIHOODS AND LANDSCAPES:V WORLD CONGRESS ON MEDICINAL AND AROMATIC PLANTS AND INTERNATIONAL SYMPOSIUM ON PLANTS AS FACTORIES OF NATURAL SUBSTANCES,EDIBLE AND ESSENTIAL OILS , 2016,:1125

DOI 10.17660/ActaHortic.2016.1125.2

Abstract Root samples of Acanthopanax trifoliatus (AT) and Acanthopanax senticosus or Siberian ginseng (AS) were investigated for phenolic compounds by high performance liquid chromatography (HPLC). The roots of AT were collected from 3 different locations in Thailand: Chiang Mai, NakhonRatchasima, and Pathum Thani. The roots of AS were supplied as a crude drug from China and as standard material from France. The root extracts of two Acanthopanax species were prepared by aqueous decoction and refluxing with 75% ethanol. Qualitative analysis by HPLC revealed that the main constituents in root samples were caffeoylquinic acid derivatives including chlorogenic acid, cynarin, 3,5-dicaffeoylquinic acid, 1,4-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid. Aqueous and 75% ethanolic root extracts from AT collected from NakhonRatchasima contained higher active constituents than those collected from other locations. The phenolic contents were 4.80 +/- 0.02 and 8.94 +/- 0.03 g 100 g(-1) of extract, respectively. These levels were similar to the phenolic contents in the aqueous and 75% ethanolic extracts of standard AS roots from France which were 4.97 +/- 0.14 and 11.63 +/- 0.08 g 100 g(-1) extract, respectively. Extracts obtained by refluxing with 75% ethanol contained higher active constituents than extracts obtained from the decoction. The results suggest that the root extracts from AT collected from NakhonRatchasima exhibit both qualitative and quantitative similarities to the phytochemical character of the root extracts from AS.

Species covered 刺五加 Acanthopanax senticosus 白簕(变种) Acanthopanax trifoliatus