PHARMACOLOGICAL EFFECTS OF DAI-SAIKO-TO ON LIPID BIOSYNTHESIS IN CULTURED HUMAN HEPATOCYTE HEPG(2) CELLS

Authors YAMAMOTO, K; OGAWA, Y; YANAGITA, T; MORITO, F; FUKUSHIMA, N; OZAKI, I; MIZUTA, T; SETOGUCHI, Y; SAKAI, T

Journal JOURNAL OF ETHNOPHARMACOLOGY , APR 1995,1:46

DOI 10.1016/0378-8741(95)01227-5 PubMed 7475122

Abstract The pharmacological effects of Dai-saiko-to, a Japanese and Chinese traditional medicinal mixture (Kampohozai), on lipid biosynthesis were investigated in cultured human hepatocyte HepG(2) cells. The addition of Dai-saiko-to (0.5 mg/ml), which had no significant effect on cell proliferation, caused a marked decrease in the intracellular triglyceride content with no significant changes in the other lipid fraction. At the same time, the incorporation of C-14-acetate or H-3-glycerol into the triglyceride or diglyceride fractions also decreased significantly. These results suggest that Dai-saiko-to decreases hepatic triglyceride biosynthesis, which might contribute to a reduction in plasma VLDL levels.