Callus Induction and Rapid Propagation Systems from a Tibetan Medicine Plant,Veronica ciliate Fisch.

Authors LI Zhan; CHEN Fang; TANG Lin

Journal Plant Science Journal , 2011,4:29

Abstract To induce callus and establish rapid propagation systems for Veronica ciliate Fisch.,terminal buds from its aseptic seedlings were cultured in MS mediums with different concentration combinations of NAA and 6-BA.Results showed that the optimum medium for callus inducement was MS+6-BA 0.5 mg/L+NAA 1.0 mg/L,with the callus induction rate of 95%.The best medium for multiplication was MS+6-BA 0.5 mg/L+NAA 0.1 mg/L,with the multiplication coefficient reaching 5.4.The optimum root medium for multiple shoot clumps was 1/2 MS+IBA 0.05 mg/L,with the rooting rate reaching 95%and the root being healthiest.Under suitable hardening and transplanting conditions,the highest survival rate for the regenerated plantlets reached 40%.