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Lucidone protects human skin keratinocytes against free radical-induced oxidative damage and inflammation through the up-regulation of HO-1/Nrf2 antioxidant genes and down-regulation of NF-kappa B signaling pathway

作者 Kumar, K. J. Senthil; Yang, Hsin-Ling; Tsai, Yu-Cheng; Hung, Pin-Chun; Chang, Show-Huei; Lo, Heng-Wei; Shen, Pei-Chun; Chen, Ssu-Ching; Wang, Hui-Min; Wang, Sheng-Yang; Chou, Chih-Wei; Hseu, You-Cheng

期刊 FOOD AND CHEMICAL TOXICOLOGY , SEP 2013,:59

DOI 10.1016/j.fct.2013.04.055 PubMed 23712098

摘要 We investigated the protective effects of lucidone, a naturally occurring cyclopentenedione isolated from the fruits of Lindera erythrocarpa Makino, against free-radical and inflammation stimulator 2,2''-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress in human keratinocyte (HaCaT) cells, with the aim of revealing the possible mechanisms underlying the protective efficacy. Lucidone pretreatment (0.5-10 mu g/mL) markedly increased HaCaT cell viability and suppressed AAPH-induced reactive oxygen species (ROS) generation, lipid peroxidation, and DNA damage. Notably, the antioxidant potential of lucidone was directly correlated with the increased expression of an antioxidant gene, heme oxygenase 1 (HO-1), which was followed by the augmentation of the nuclear translocation and transcriptional activation of NF-E2-related factor-2 (Nrf2), with or without AAPH. Nrf2 knockdown diminished the protective effects of lucidone. We also observed that lucidone pretreatment inhibited AAPH-induced inflammatory chemokine prostaglandin E-2 (PGE(2)) production and the expression of cyclooxygenase-2 (COX-2) in HaCaT cells. Lucidone treatment also significantly inhibited AAPH-induced nuclear factor-kappa B (NF-kappa B) activation and suppressing the degradation of inhibitor-kappa B (I-kappa B). Furthermore, lucidone significantly diminished AAPH-induced COX-2 expression through the down-regulation of the extracellular signal-regulated kinase (ERK) and p38 MAPK signaling pathways. Therefore, lucidone may possess antioxidant and anti-inflammatory properties and may be useful for the prevention of free radical-induced skin damage. (c) 2013 Elsevier Ltd. All rights reserved.

涉及物种 红果山胡椒 Lindera erythrocarpa