Detection of Leifsonia xyli subsp. xyli,causal bacterium of sugarcane ratoon stunting disease,from fruit cane planting areas in Guangdong

Authors Dou Zhimin; Pan Lingling; Deng Quanqing; Wang Kaili; Chen Peishou; Shen Wankuan

Journal Plant Protection , 2017,6:43

Abstract This study aimed to understand the occurrence situation of ratoon stunting disease (RSD) in Guangdong fruit cane planting areas and provide scientific basis for the production and application of healthy seedlings. Conventional PCR and nested PCR were used to detect Leifsonia xyli subsp. xyli,the causal bacterium of ratoon stunting disease,from fruit cane cultivars,including ''Badila'',''Neijiang mizhe'',''Tiancheng No.21'',''Tiancheng No.22'' and ''Tiancheng No.99''. Samples of ''Badila'' used as the main fruit cane cultivar were collected from Shaoguan City and Nansha District of Guangdong Province. Samples of introduced fruit cane cultivars ''Neijiang mizhe'',''Tiancheng No.21'',''Tiancheng No.22'' and ''Tiancheng No.99'' were collected from the sugarcane breeding base of South China Agricultural University (SCAU). The results showed that the positive detection rate was 88.6% by nested PCR,significantly higher than that by conventional PCR,which was 40.4%. The RSD positive rate of ''Badila'' was 86.8% in Shaoguan and 92.7% in Nansha of Guangzhou,respectively. The introduced fruit cane cultivars from the sugarcane breeding base of SCAU were all infected by RSD pathogen except the ''Tiancheng No.22''. The sugarcane ratoon stunting disease has occurred widely in the major fruit cane planting areas of Guangdong. It is necessary to carry out the research and application of healthy fruit cane seedlings.