Metabolomics Analysis of Seed Dormancy Release of Traditional Chinese Medicine Plant Notopterygium incisum

Authors Li Aihua; Jiang Shunyuan; Guo Na; Huang Luqi

Journal Journal of Agricultural Science and Technology , 2021,5:23

Abstract The seeds of Notopterygium incisum,a traditional Chinese medicine plant,are dormant,which limits its artificial domestication and cultivation.In order to analyze the metabolite changes and screen the key compounds during releasing dormancy of N.incisum seeds,seeds of N.incisum were pretreated with cold and warm stratification;and the pretreated seeds were treated with low temperature stratification (CS),ABA inhibitor fluridone (FL) and GA3(GA),respectively.Then seeds of different treatments were detected with UPLC-MS/MS.The results showed that the whole treated process of N.incisum seeds could be divided into three stages:060 d,90(150+30) d and(150+60) (150+110) d,among which N.incisum seeds treated with pretreatment+CS for (150+60) and (150+ 110) d had germination capablity.The compounds represented by the principal component t[1]were the compounds that had undergone major changes of seed embryos during 0,15,60,90,120 d after pretreatments.The compounds represented by the principal component of t[2] were mainly changed during the release of physiological dormancy.The difference analysis showed that the content of orobanchyl acetate,a strigolactone compound,was accumulated significantly in released dormancy of N.incisum seed,which was increased by 12.1 times after FL treatment and 28.5 times after CS treatment.Therefore,orobanchyl acetate could be crucial during the dormancy release of N.incisum seeds.This study provided a theoretical basis for dormancy release mechanism of N.incisum seeds and a practical significance for the development of Chinese medicine industry of N.incisum.

Species covered 羌活 Notopterygium incisum