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Bioassay-guided isolation of anti-seizure principles from Semen Pharbitidis using a zebrafish pentylenetetrazol seizure model

Authors Liu, Maoxuan; Copmans, Danielle; Lu, Jing-Guang; Yang, Ming-Rong; Sourbron, Jo; Ny, Annelii; Jiang, Zhi-Hong; de Witte, Peter A. M.; Luyten, Walter

Journal JOURNAL OF ETHNOPHARMACOLOGY , MAR 25 2019,:232

DOI 10.1016/j.jep.2018.12.024 PubMed 30572093

Abstract Ethnopharmacological relevance: Semen Pharbitidis, the seeds of Pharbitis nil (Linn.) Choisy (Convolvulaceae) is a well-known traditional Chinese medicinal plant used for treating helminthiasis and epilepsy in China.Aim of the study: This study aims to identify the anti-seizure components from Semen Pharbitidis.Methods: A bioassay-guided isolation of anti-seizure compounds from Semen Pharbitidis was performed using a zebrafish pentylenetetrazol seizure model. The structures of active compounds were elucidated by high resolution mass spectrometry. The fragments of active compounds were tested for anti-seizure activity as well.Results: The bioassay-guided isolation of ethanol extract of Semen Pharbitidis led to a group of resin glucosides, namely pharbitin. One of the fragments of pharbitin, 2-methylbutyric acid, also showed anti-seizure activity.Conclusions: We provided further experimental scientific evidence to support the traditional use of Semen Pharbitidis for the treatment of epilepsy. Pharbitin was identified to be the main anti-seizure component in Semen Pharbitidis.

Species covered 牵牛 Pharbitis nil