RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS (RFLPS) OF MEDICINAL-PLANTS AND CRUDE DRUGS .1. RFLP PROBES ALLOW CLEAR IDENTIFICATION OF DUBOISIA INTERSPECIFIC HYBRID GENOTYPES IN BOTH FRESH AND DRIED TISSUES

Authors MIZUKAMI, H; OHBAYASHI, K; KITAMURA, Y; IKENAGA, T

Journal BIOLOGICAL & PHARMACEUTICAL BULLETIN , APR 1993,4:16

PubMed 8102923

Abstract A simple and efficient method for identification of Duboisia leichhardtii F. MUELL, D. myoporoides R. BR. and their interspecific hybrid was established by analyzing restriction fragment length polymorphism (RFLP) profiles using non-radioactive rice ribosomal DNA as a probe. This procedure was shown to be applicable to both fresh and dried samples, and also suitable for detection of somatic hybrid at the callus stage after protoplast fusion.