Relationships between pharmacokinetics and efficacy of Xie-xin decoction in rats with experimental ulcerative colitis

Authors Han, Xiang-Hui; Zhong, Jie; Guo, Ji-Yuan; Shi, Rong; Wang, Xin-Hong; Wang, Chang-Hong; Wang, Kun; Du, Guang-Li; Shen, Yun-Hui; Ma, Yue-Ming

Journal JOURNAL OF ETHNOPHARMACOLOGY , JUN 21 2013,1:148

DOI 10.1016/j.jep.2013.04.008 PubMed 23619018

Abstract Ethnopharmacological relevance: Xie-xin decoction (XXD) has been used as a classic formula in China for the treatment of gastrointestinal dysfunction such as ulcerative colitis (UC). However, no potential action mechanisms and active compounds had been systematically investigated.Aim of the study: To explore the effectiveness and the material basis of XXD in trinitrobenzene sulfonic acid (TNBS)-induced UC rats.Materials and methods: XXD was administered orally for 8 days at a dosage of 2 or 4 g/kg/day. Plasma pharmacokinetic properties and colon tissue concentrations of multiple compounds from XXD were detected. Tissue damage scores, production of interleukin (IL)-10 and myeloperoxidase (MPO), expression of tumor necrosis factor-alpha (TNF-alpha) and nuclear factor-kappa Bp65 (NF-kappa Bp65) in colon tissues were examined. Canonical correlation analysis was performed to evaluate the relationships between pharmacokinetics and efficacy to elucidate significantly active compounds of XXD.Results: XXD promoted the recovery of colitis and inhibited the colonic inflammation damage in UC rats by reducing the level of MPO and the expression of TNF-alpha and NF-kappa Bp65, and increasing the production of IL-10 in colon tissues. Efficacy of XXD was positively related with AUC of five plasma compounds (baicalin, berberine, wogonoside, wogonin, and rhein) and concentrations of six colon tissue compounds (coptisine, jatrorrhizine, palmatine, berberine, baicalein and emodin), respectively.Conclusions: The multiple compounds in plasma and colon tissues from XXD might be the main material basis for therapeutic potentials in UC rats. (C) 2013 Elsevier Ireland Ltd. All rights reserved.