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Modulation effect of Smilax glabra flavonoids on ryanodine receptor mediated intracellular Ca2+ release in cardiomyoblast cells

Authors Shou, Qiyang; Pan, Shuizhen; Tu, Jue; Jiang, Jianping; Ling, Yun; Cai, Yueqin; Chen, Minli; Wang, Dejun

Journal JOURNAL OF ETHNOPHARMACOLOGY , OCT 28 2013,1:150

DOI 10.1016/j.jep.2013.08.009 PubMed 23954280

Abstract Ethnopharmacological relevance: Smilax glabra rhizome, a plant material from Liliaceae family, is a widely used traditional Chinese medicine for anti-cardiac hypertrophy treatment. We have previously found that Smilax glabra flavonoids (SGF) exerted such anti-cardiac hypertrophy activity. However, the mechanism of this activity of SGF has not been clarified yet.Materials and methods: This study was aimed to investigate the inhibitory role of SGF on intracellular Ca2+ release in rat cardiomyoblast cells (H9C2). Intracellular Ca2+ release was determined by Ca2+ indicator fluorescence (fluo 4-AM) in H9C2 cell line.Results: SGF at concentrations of 0.25, 0.5, 1.0 mg/ml significantly inhibited the phenylephrine or angiotensin II induced intracellular Ca2+ release in a dose-dependent manner. Furthermore, SGF could also inhibit ryanodine receptor (RyR) agonist caffeine induced Ca2+ release and phenylephrine (PE)-induced Ca2+ release under the condition in which inositol trisphosphate (IP3) receptors were blocked with 2-Aminoethoxydiphenyl borate (2-APB). Nevertheless, SGF had no impact on PE-induced Ca2+ release under the condition in which RyRs were blocked with tetracaine.Conclusions: Our results suggest that the protective effects of SGF are mediated via targeting inhibition of RyR mediated intracellular Ca2+ release. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

Species covered 土茯苓 Smilax glabra