SYOSAIKO-TO AND SAIBOKU-TO (CHINESE-JAPANESE HERBAL MEDICINE) SUPPRESS THE RELEASE OF ARACHIDONIC-ACID METABOLITES FROM CULTURED PORCINE PULMONARY-ARTERY ENDOTHELIAL-CELLS

Authors WATANABE, K; TAMARU, N; TAKIHARA, H; YOSHIDA, M

Journal JOURNAL OF ETHNOPHARMACOLOGY , JUL 22 1994,3:43

DOI 10.1016/0378-8741(94)90042-6 PubMed 7990493

Abstract The influences of Syosaiko-to and Saiboku-to, traditional Chinese-Japanese medicines, on the arachidonic acid metabolism of porcine pulmonary artery endothelial cells (PPAEC) in culture were investigated. 6-keto-PGF(1 alpha) a stable end product of prostacyclin, released by PPAEC during a 48 h incubation period with 1000 mu g/ml of Syosaiko-to or Saiboku-to was significantly decreased. Analysis of the eicosanoid profile by high performance liquid chromatography revealed that both cyclooxygenase and lipoxygenase derivatives were released in smaller than usual proportions from PPAEC during 24 h incubation periods with 500 mu g/ml of Syosaiko-to or Saiboku-to. Clinically, Syosaiko-to is used for chronic hepatitis and Saiboku-to is prescribed for bronchial asthma. The experimental findings could explain the anti-inflammatory effect of Syosaiko-to and Saiboku-to from the perspective of their influences on arachidonic acid metabolic activity associated with PPAEC.