Dangguijakyak-san, a medicinal herbal formula, protects dopaminergic neurons from 6-hydroxydopamine-induced neurotoxicity

作者 Hwang, Deok-Sang; Kim, Hyo Geun; Kwon, Ha-Jeong; Cho, Jung-Hoon; Lee, Chang-Hoon; Lee, Jin-Moo; Jang, Jun-Bock; Kim, Yong-Suk; Lee, Kyung-Sub; Oh, Myung Sook

期刊 JOURNAL OF ETHNOPHARMACOLOGY , JAN 27 2011,2:133

DOI 10.1016/j.jep.2010.11.017 PubMed 21075188

摘要 Aim of the study: Dangguijalcyak-san (DJS) is a multi-herbal formula that has long been widely used in traditional Oriental medicine to treat gynecologic disorders, including neurological symptoms. Recent clinical and experimental studies have reported aging and anti-neurodegenerative effects of DJS. In this study, we evaluated the neuroprotective effects of DJS on dopaminergic (DA) neurons damaged by 6-hydroxydopamine (6-OHDA).Materials and methods: To evaluate the protective effects of DJS, we analyzed viability in SH-SY5Y neuroblastoma cells and tyrosine hydroxylase (TH) staining in primary DA cells. To explore the possible mechanism(s) of neuroprotection, we assessed anti-oxidant activity by measuring reactive oxygen species (ROS) and glutathione (GSH) levels. To determine mitochondria-mediated apoptotic activity, we examined mitochondrial membrane potential, cytochrome c release, and caspase-3 activation.Results: DJS at 0.05-5 mu g/mL significantly protected SH-SY5Y cells from 6-OHDA toxicity, dose-dependently, and attenuated 6-OHDA damage in primary DA cells. DJS reduced 6-OHDA-induced intracellular ROS production and GSH depletion and inhibited mitochondrial membrane instability, cytosolic cytochrome c release, and caspase-3 activation.Conclusions: These results demonstrate that DJS has neuroprotective effects in DA neurons against 6-OHDA-induced toxicity through anti-oxidant and anti-mitochondrial-mediated apoptotic activities. (C) 2010 Elsevier Ireland Ltd. All rights reserved.