Urinary metabonomics study on toxicity biomarker discovery in rats treated with Xanthii Fructus

作者 Lu, Fang; Cao, Min; Wu, Bin; Li, Xu-zhao; Liu, Hong-yu; Chen, Da-zhong; Liu, Shu-min

期刊 JOURNAL OF ETHNOPHARMACOLOGY , AUG 26 2013,1:149

DOI 10.1016/j.jep.2013.06.040 PubMed 23831081

摘要 Ethnopharmacological relevance: Xanthii Fructus (XF) is commonly called Cang-Erzi in traditional Chinese medicine (TCM) and widely used for the treatment of sinusitis, headache, rheumatism, and skin itching. However, the clinical utilization of XF is relatively restricted owing to its toxicity. Aim of the study: To discover the characteristic potential biomarkers in rats treated with XF by urinary metabonomics.Materials and methods: Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) was applied in the study. The total ion chromatograms obtained from control and different dosage groups were distinguishable by a multivariate statistical analysis method.Results: The greatest difference in metabolic profile was observed between high dosage group and control group, and the metabolic characters in rats treated with XF were perturbed in a dose-dependent manner. The metabolic changes in response for XF treatment were observed in urinary samples, which were revealed by orthogonal projection to latent structures discriminate analysis (OPLS-DA), and 10 metabolites could be served as the potential toxicity biomarkers. In addition, the mechanism associated with the damages of lipid per-oxidation and the metabolic disturbances of fatty acid oxidation were investigated.Conclusions: These results indicate that metabonomics analysis in urinary samples may be useful for predicting the toxicity induced by XF. (C) 2013 Elsevier Ireland Ltd. All rights reserved.