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PREPARATIVE ISOLATION AND PURIFICATION OF THREE FLAVONOIDS FROM THE CHINESE MEDICINAL PLANT ALPINIA KATSUMADAI HAYATA BY HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY

作者 Li, Aifeng; Sun, Ailing; Liu, Renmin

期刊 JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES , 2012,20:35

DOI 10.1080/10826076.2011.643523

摘要 High-speed counter-current chromatography was applied in the isolation and purification of three flavonoids from the Chinese medicinal plant Alpinia katsumadai Hayata. A two-phase solvent system composed of n-hexaneethyl acetatemethanolwater at volume ratios of 5:5:7:3 was used, which yielded 17.2 mg of alpinetin, 3.5 mg of pinocembrin, and 25.1 mg of ardamomin from 100 mg of the crude extract in one-step separation. The purities of them were 98.1%, 97.5%, and 99.2%, respectively, as determined by high performance liquid chromatography and their chemical structures were identified by 1H-NMR and 13C-NMR.

涉及物种 草豆蔻 Alpinia katsumadai