Butanol fraction of Khaya senegalensis root modulates beta-cell function and ameliorates diabetes-related biochemical parameters in a type 2 diabetes rat model

作者 Ibrahim, Mohammed Auwal; Islam, Md Shahidul

期刊 JOURNAL OF ETHNOPHARMACOLOGY , JUL 3 2014,3:154

DOI 10.1016/j.jep.2014.05.011 PubMed 24846204

摘要 Ethnopharmacological relevance: Khaya senegalensis A. Juss (Meliaceae) is commonly exploited for the traditional treatment of diabetes mellitus in Nigeria and Togo. The present study was conducted to examine the anti-diabetic activity of Khaya senegalensis butanol fraction (KSBF) of root ethanolic extract in a type 2 diabetes (T2D) model of rats.Materials and methods: T2D was induced in rats by feeding a 10% fructose solution ad libitum for two weeks followed by a single intraperitoneal injection of streptozotocin (40 mg/kg body weight) and the animals were treated with 150 and 300 mg/kg body weight (BW) of the fraction for five days in a week. Relevant diabetes-related parameters were analyzed in all experimental animals.Results: The KSBF treatment, at 300 mg/kg BW, significantly (p < 0.05) reduced blood glucose level, improved oral glucose tolerance ability and beta-cell function (HOMA-beta), decreased insulin resistance (HOMA-IR), stimulated hepatic glycogen synthesis, ameliorated serum lipids alterations and prevented hepatic and renal damages compared to untreated diabetic rats. Additionally, the fraction insignificantly (p > 0.05) improved weight gain, decreased food and fluid intake, stimulated insulin secretion and lowered serum fructosamine concentrations compared to untreated diabetic rats.Conclusions: Data from this study suggests that orally administered KSBF, at 300 mg/kg BW, possess remarkable anti-type 2 diabetic activity and could ameliorate some diabetes-associated complications and hence can be considered as a source of potential anti-type 2 diabetic medicine. (C) 2014 Elsevier Ireland Ltd. All rights reserved.