Endophytic fungi from Dracaena cambodiana and Aquilaria sinensis and their antimicrobial activity

Authors Gong, Li-Juan; Guo, Shun-Xing

Journal AFRICAN JOURNAL OF BIOTECHNOLOGY , MAR 6 2009,5:8

Abstract Single mycelium method was used to isolate endophytic fungi from surface-sterilized tissues of two medicinal plants. Of the 300 isolates recovered, 172 were from Dracaena cambodiana and 128 from Aquilaria sinensis. According to morphological characteristics, 174 (58%) isolates were identified and belonged to 41 taxa in 30 genera. The remaining 126 (42%) isolates did not sporulate and were segregated into 8 morphological groups. There were colonization rates (34 - 80%) and isolation rates (0.62 - 2.06) in two plants. And some endophytes showed certain level of host specificity or organs specificity. 21 (8.3%) isolates showed antimicrobial activity, moreover, some of them exhibited broad-spectrum antimicrobial activity and the inhibition zones ranged from 7 to 27 mm. The active isolates were identified to 17 taxa. Fusarium spp. were the most dominant genera in two plants and showed the most potent antimicrobial activity.

Species covered 柬埔寨龙血树 Dracaena cambodiana 土沉香 Aquilaria sinensis