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Study on separation of aristolochic acid I and II by micellar electrokinetic capillary chromatography and competition mechanism between SDS and beta-cyclodextrin

作者 Li, W; Chen, Z; Liao, Y; Liu, H

期刊 ELECTROPHORESIS , FEB 2006,4:27

DOI 10.1002/elps.200500228 PubMed 16411270

摘要 In this study, a rapid MEKC method using 40mM sodium borate buffer containing 50 mM SDS as surfactant was developed for the analysis of aristolochic acid (AA) in Aristolochia plants. Baseline separation of AA-I and AA-II was achieved within 3 min with high separation efficiency, satisfactory sensitivity, repeatability, and recovery. Resolution between AA-I and AA-II is above 5 and great performance with higher than 200 000 theoretical plate numbers was obtained. The detection limits (based on 3 S/N) were both 1.0 mu g/mL. Two kinds of AA in 35 herbal samples of Aristolochia plants were successfully determined. The competition mechanism between P-CD and SDS was also investigated by changing the content ratio of P-CD and SDS.

涉及物种 马兜铃属 Aristolochia