Distribution of ARGs and MGEs among glacial soil, permafrost, and sediment using metagenomic analysis

Authors Zhang, Shuhong; Yang, Guangli; Hou, Shugui; Zhang, Tingjun; Li, Zhiguo; Liang, Feng

Journal ENVIRONMENTAL POLLUTION , MAR 2018,:234

DOI 10.1016/j.envpol.2017.11.031 PubMed 29195175

Abstract Antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) can be identified with metagenomic analyses comparing relatively pristine and human-impacted environments. We collected samples from 3 different environments: glacial soil little affected by anthropogenic activity, deep permafrost dated to 5821 BP (before human antibiotics), and sediment from the Pearl River. Sulfonamides, tetracyclines, and fluoroquinolones were common in the sediment samples. Sulfonamides and tetracycline were not found in permafrost; tetracycline was also not found in glacial soil. ARGs from the sediment were more abundant and diverse than those from glacial soil and permafrost. More types of resistance mechanisms were also present in the sediment. The diversity of MGEs was significantly correlated with the abundance and diversity of ARGs. The result will help future workers to better understand the distribution of ARGs among environments more or less impacted by anthropogenic activities. (C) 2017 Elsevier Ltd. All rights reserved.