Study on physiological changes of calyx in Clerodendrum trichotomum Thunb

Authors Yang Xiulian; Hua Yajie; Lu Chenyan; Wang Lianggui

Journal Journal of Northwest A & F University. Natural Science Edition , 2019,4:47

Abstract 【Objective】To explore the composition and dynamic changes of the calyx pigment in Clerodendrum trichotomum.【Method】Six Clerodendrum trichotomum samples(Tinghu,Yancheng;Jurong,Zhenjiang;Yuntaishan,Lianyungang;Jiawang,Xuzhou;Taishan,Taian;Dongguanzhuang,Zhumadian) from different provenances introduced into Nanjing were used as experimental materials to detect pigment composition in the calyx through identifying the anthocyanin components using color reaction,UV-VIS spectroscopy and HPLC method.At the six developmental stages(primary full-blooming stage,middle fullblooming stage,flower and fruit coexistence stage,fruit-bearing stage,fruit and calyx persistent stage,defoliating stage)sampling,mesuring the changes of chlorophyll,anthocyanin,soluble sugar and anthocyanidin synthase in the process of calyx discoloration,meanwhile analyzing the correlation between anthocyanins and other indicators.【Result】It showed that there were chlorophyll,anthocyanin and flavonoids,no carotenoids in the Clerodendrum trichotomum calyx.A total of 3 anthocyanin constituents were identified in which the Cyanidin-3-O-glucoside was confirmed.During the process of color change,the content of chlorophyll decreased,the content of anthocyanin and soluble sugar increased,PAL enzyme activity fluctuated greatly at the later color-turning stage and the activity of CHI enzyme increased obviously.Through correlation analysis found that anthocyanin content was significantly correlated with chlorophyll content,soluble sugar content,PAL and CHI enzyme activities.【Conclusion】Change in CHI enzyme during color conversion was the main cause of calyx color change in Clerodendrum trichotomum.

Species covered 海州常山 Clerodendrum trichotomum